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actin tracker green 488  (Beyotime)


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    Structured Review

    Beyotime actin tracker green 488
    Actin Tracker Green 488, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/actin+tracker+green+488/Actin-Tracker+Green-488/pm42901393-76-17-19
    Average 99 stars, based on 587 article reviews
    actin tracker green 488 - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Metabolic‐Redox Remodeling of Inflammatory Synovium by Mannose‐Modified Selenium Nanoparticles for Osteoarthritis Therapy
    Article Snippet: .. Cells were washed with PBS, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X‐100, and stained with Actin‐Tracker Green‐488 (1:200, Beyotime) and DAPI (1:1000, CST). .. Cellular internalization was observed via a Leica STELLARIS 5 confocal microscope and quantitatively assessed by flow cytometry (BD LSRFortessa) with DiD fluorescence measured in the APC channel.

    Article Title: Hyperoside-based self-assembled hydrogel for modulating osteoimmune microenvironment to combat bacterial infections and facilitate bone repair
    Article Snippet: Recombinant murine interleukin-4 (IL-4, RP01465) and interleukin-10 (IL-10, RP01161) were obtained from ABclonal Technology Co., Ltd. (Wuhan, China). .. Live/Dead Bacteria Staining Kit (C2030S), Cell Counting Kit-8 (CCK-8, C0038), BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 594 (C0078S), Calcein-AM/Propidium Iodide (PI) Cell Viability/Cytotoxicity Assay Kit (C2015S), Actin-Tracker Red-594 (C2237-5 mL), Actin-Tracker Green-488 (C2201S), Improved Citrate Antigen Retrieval Solution (P0083), and Immunol Staining Blocking Buffer (P0102) were all purchased from Beyotime Biotechnology Co. (Jiangsu, China). .. The MRSA strain (ATCC BAA-1717) was obtained from Bio Sci (Zhejiang, China).

    Article Title: Hydrogen inhalation attenuates mechanical ventilation-induced postoperative cognitive dysfunction in aged mice by inhibiting the lung–brain axis-mediated transmission of pTau217-enriched extracellular vesicles
    Article Snippet: .. The cellular cytoskeleton was stained using Actin-Tracker Green-488 (Beyotime, C2232), and the nuclei were counterstained with DAPI-containing mounting medium. .. EV internalization was visualized and examined using a Zeiss LSM980 confocal laser scanning microscope.

    Article Title: Empagliflozin-pretreated mesenchymal stem cell-derived extracellular vesicles facilitate cardiac repair via AGGF1
    Article Snippet: To verify their uptake ability, EVs were labeled with the red fluorescent dye Dil (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate, Beyotime, C1036), and the unbound dye was removed. .. Then, the EVs were co-incubated with H9c2 rat cardiomyocytes, HUVECs, and mouse monocyte - macrophage leukemia cell line RAW 264.7 cells for 24 h. Subsequently, the cytoskeleton was stained with Actin-Tracker Green-488 (Beyotime, C2201S), and the nuclei with DAPI (4′,6 - diamidino −2 - phenylindole, Beyotime, C1005). ..

    Article Title: Apatinib attenuates CRKII-Mediated angiogenesis: Possible implications for metastasis prevention from small extracellular vesicle proteomics.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Young plasma-derived exosomes deliver YOD1 to induce M2 polarization and ameliorate osteoporosis via mTOR signaling pathways
    Article Snippet: The primary antibodies were as follows: CD86 Rabbit antibody (Immunoway, USA, YM8023, 1:100), CD206 Rabbit antibody (Immunoway, USA, YM8349, 1:100), iNOS Rabbit antibody (Proteintech, China, 22226-1-AP, 1:100), and ARG1 Rabbit antibody (Abcam, China, ab96183, 1:100). .. After incubation, cells were washed three times with PBST, then incubated with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Invitrogen, A-11012, 1:2000) and Actin-Tracker Green-488 (Beyotime, C2201S, 1:200) at room temperature for 2 h. After that, cells were washed and incubated with DAPI staining solution (MedChemExpress, China, HY-D0814) at room temperature in the dark for 10 min. After thorough washing with PBS, cells were observed under a fluorescence microscope (IX73, Olympus Corporation, Japan). ..

    Bacteria:

    Article Title: Hyperoside-based self-assembled hydrogel for modulating osteoimmune microenvironment to combat bacterial infections and facilitate bone repair
    Article Snippet: Recombinant murine interleukin-4 (IL-4, RP01465) and interleukin-10 (IL-10, RP01161) were obtained from ABclonal Technology Co., Ltd. (Wuhan, China). .. Live/Dead Bacteria Staining Kit (C2030S), Cell Counting Kit-8 (CCK-8, C0038), BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 594 (C0078S), Calcein-AM/Propidium Iodide (PI) Cell Viability/Cytotoxicity Assay Kit (C2015S), Actin-Tracker Red-594 (C2237-5 mL), Actin-Tracker Green-488 (C2201S), Improved Citrate Antigen Retrieval Solution (P0083), and Immunol Staining Blocking Buffer (P0102) were all purchased from Beyotime Biotechnology Co. (Jiangsu, China). .. The MRSA strain (ATCC BAA-1717) was obtained from Bio Sci (Zhejiang, China).

    CCK-8 Assay:

    Article Title: Hyperoside-based self-assembled hydrogel for modulating osteoimmune microenvironment to combat bacterial infections and facilitate bone repair
    Article Snippet: Recombinant murine interleukin-4 (IL-4, RP01465) and interleukin-10 (IL-10, RP01161) were obtained from ABclonal Technology Co., Ltd. (Wuhan, China). .. Live/Dead Bacteria Staining Kit (C2030S), Cell Counting Kit-8 (CCK-8, C0038), BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 594 (C0078S), Calcein-AM/Propidium Iodide (PI) Cell Viability/Cytotoxicity Assay Kit (C2015S), Actin-Tracker Red-594 (C2237-5 mL), Actin-Tracker Green-488 (C2201S), Improved Citrate Antigen Retrieval Solution (P0083), and Immunol Staining Blocking Buffer (P0102) were all purchased from Beyotime Biotechnology Co. (Jiangsu, China). .. The MRSA strain (ATCC BAA-1717) was obtained from Bio Sci (Zhejiang, China).

    Blocking Assay:

    Article Title: Hyperoside-based self-assembled hydrogel for modulating osteoimmune microenvironment to combat bacterial infections and facilitate bone repair
    Article Snippet: Recombinant murine interleukin-4 (IL-4, RP01465) and interleukin-10 (IL-10, RP01161) were obtained from ABclonal Technology Co., Ltd. (Wuhan, China). .. Live/Dead Bacteria Staining Kit (C2030S), Cell Counting Kit-8 (CCK-8, C0038), BeyoClick EdU Cell Proliferation Kit with Alexa Fluor 594 (C0078S), Calcein-AM/Propidium Iodide (PI) Cell Viability/Cytotoxicity Assay Kit (C2015S), Actin-Tracker Red-594 (C2237-5 mL), Actin-Tracker Green-488 (C2201S), Improved Citrate Antigen Retrieval Solution (P0083), and Immunol Staining Blocking Buffer (P0102) were all purchased from Beyotime Biotechnology Co. (Jiangsu, China). .. The MRSA strain (ATCC BAA-1717) was obtained from Bio Sci (Zhejiang, China).

    Article Title: Apatinib attenuates CRKII-Mediated angiogenesis: Possible implications for metastasis prevention from small extracellular vesicle proteomics.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Labeling:

    Article Title: TiO2 nanowhiskers functionalized with organic-inorganic hybrid SiO2 coating for reinforcing PEEK: A dual-pronged strategy to develop nanocomposites with enhanced stiffness, strength, toughness and osteogenesis for load-bearing implant
    Article Snippet: Polyetheretherketone (PEEK) used in load-bearing orthopedic implants faced limitations in mechanical strength and osteogenic activity.. Current reinforcement strategies still faced dilemma, including: fiber incorporation resulted in compromised osteogenicity and antibacterial property; bulk modification with bioactive fillers faced dramatically reduced strength and toughness; surface modification with bioactive coatings encountered lack of mechanical enhancement and coating delamination, leaving the clinical potential of PEEK unmet.. Herein, we reported a multifunctional bioactive shell coated whisker-reinforcing strategy: a novel titanium dioxide nanowhisker coated with amino-functionalized organic-inorganic hybrid silica shell (CRT) designed for reinforcing PEEK to simultaneously achieve enhanced stiffness, toughness and cortical bone-matching modulus, alongside improved osteogenic properties.

    Incubation:

    Article Title: Young plasma-derived exosomes deliver YOD1 to induce M2 polarization and ameliorate osteoporosis via mTOR signaling pathways
    Article Snippet: The primary antibodies were as follows: CD86 Rabbit antibody (Immunoway, USA, YM8023, 1:100), CD206 Rabbit antibody (Immunoway, USA, YM8349, 1:100), iNOS Rabbit antibody (Proteintech, China, 22226-1-AP, 1:100), and ARG1 Rabbit antibody (Abcam, China, ab96183, 1:100). .. After incubation, cells were washed three times with PBST, then incubated with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Invitrogen, A-11012, 1:2000) and Actin-Tracker Green-488 (Beyotime, C2201S, 1:200) at room temperature for 2 h. After that, cells were washed and incubated with DAPI staining solution (MedChemExpress, China, HY-D0814) at room temperature in the dark for 10 min. After thorough washing with PBS, cells were observed under a fluorescence microscope (IX73, Olympus Corporation, Japan). ..

    Fluorescence:

    Article Title: Young plasma-derived exosomes deliver YOD1 to induce M2 polarization and ameliorate osteoporosis via mTOR signaling pathways
    Article Snippet: The primary antibodies were as follows: CD86 Rabbit antibody (Immunoway, USA, YM8023, 1:100), CD206 Rabbit antibody (Immunoway, USA, YM8349, 1:100), iNOS Rabbit antibody (Proteintech, China, 22226-1-AP, 1:100), and ARG1 Rabbit antibody (Abcam, China, ab96183, 1:100). .. After incubation, cells were washed three times with PBST, then incubated with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Invitrogen, A-11012, 1:2000) and Actin-Tracker Green-488 (Beyotime, C2201S, 1:200) at room temperature for 2 h. After that, cells were washed and incubated with DAPI staining solution (MedChemExpress, China, HY-D0814) at room temperature in the dark for 10 min. After thorough washing with PBS, cells were observed under a fluorescence microscope (IX73, Olympus Corporation, Japan). ..

    Microscopy:

    Article Title: Young plasma-derived exosomes deliver YOD1 to induce M2 polarization and ameliorate osteoporosis via mTOR signaling pathways
    Article Snippet: The primary antibodies were as follows: CD86 Rabbit antibody (Immunoway, USA, YM8023, 1:100), CD206 Rabbit antibody (Immunoway, USA, YM8349, 1:100), iNOS Rabbit antibody (Proteintech, China, 22226-1-AP, 1:100), and ARG1 Rabbit antibody (Abcam, China, ab96183, 1:100). .. After incubation, cells were washed three times with PBST, then incubated with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Invitrogen, A-11012, 1:2000) and Actin-Tracker Green-488 (Beyotime, C2201S, 1:200) at room temperature for 2 h. After that, cells were washed and incubated with DAPI staining solution (MedChemExpress, China, HY-D0814) at room temperature in the dark for 10 min. After thorough washing with PBS, cells were observed under a fluorescence microscope (IX73, Olympus Corporation, Japan). ..



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